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High - resolution polyacrylamide gradient gel electrophoresis (PGGE) was used to separate isoenzymes of Acanthamoeba culbertsoni, which is etiological agents of primary amoebic meningoencephalilis. Of the six enzymes studied, propionyl esterase and malate dehydrog-enase showed 4 bands, acid phosphatase and alkaline phosphatase showed 2 bands, leucine aminopeptidase and glutamate dehydrogenase showed 1 band, respectively. The PGGE technique, which separates isoenzymes by their molecular shape, is both sensitive and economical. It offers an addition or an attractive alternative to isoelectric focusing which has commonly been used to aid species identification of free-living amoeba by separating isoenzymes by their isoelectric point.
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